The clinicopathologic and genetic analysis of lung cancers with ALK translocation

针对ALK易位的肺癌，我们试图建立合适的检测EML 4-ALK肺癌的方法，检测其新的变异体，分析其临床病理和遗传学特征，并寻找其他ALK易位的肺癌。我们通过cDNA筛选和多重RT-PCR鉴定了EML-ALK融合基因的新变体。我们发现EML 4-ALK肺癌的特征是罕见的其他突变、TTF-1细胞谱系、低分化腺泡组织学和粘蛋白、年轻发病和非吸烟或轻度吸烟状态。此外，通过开发一种适用于检测ALK易位的灵敏免疫组织化学方法，我们在肺癌中发现了一种新的融合癌激酶KIF 5 B-ALK。

Focusing on lung cancers with ALK translocation, we have been attempting to establish the appropriate method to detect EML4-ALK lung cancers, to detect their new variants, to analyze the clinicopathologic and genetic characteristics of them, and to find out the other ALK translocated lung cancers. We have identified novel variants for EML-ALK fusion gene with cDNA screening and multiplex RT-PCR. We have found out that EML4-ALK lung cancers are characterized by rare other mutations, TTF-1 cell lineage, less-differentiated acinar histology with mucin, young onset, and non- or light smoking status. Furthermore, by developing a sensitive immunohistochemical method appropriate for detecting ALK translocation, we have discovered KIF5B-ALK, a novel fusion oncokinase, in lung cancers.