Altered renal medullary gene expression to regulate pressure-natriuresis in diabetic hypertensive rats

研究糖尿病高血压大鼠(OLETF大鼠)及其对照大鼠(LETO大鼠)氧化应激和成骨相关基因的表达。实时定量逆转录聚合酶链式反应分析骨形态发生蛋白2(BMP2)、核心结合因子1(Cbfa1)、基质玻璃蛋白(MGP)、碱性磷酸酶(ALP)、骨羧酸谷氨酸蛋白2(Bglap2)、过氧化氢酶(CAT)、Gp91Phox、p22Phox和klotho基因在肾髓质和皮质中的表达。与LETO大鼠相比，OLETF大鼠肾皮质BMP2、cbfa1、MGP和ALP基因表达增加。与LETO大鼠相比，OLETF大鼠肾髓质Klotho mRNA表达降低。OLETF和LETO大鼠肾脏髓质和皮质过氧化氢酶基因表达差异无统计学意义，这可能是糖尿病高血压大鼠血压-血钠右移和心血管钙化增强的原因之一。

Gene expressions related to oxidative stress and osteogenesis were investigated in diabetic hypertensive rats (OLETF rats) and its control rats (LETO rats). Gene expressions of bone morphogenetic protein 2 (BMP2), core binding factor 1 (cbfa1), matrix gla protein (MGP), alkaline phosphatase (Alp), bone gamma carboxyglutamate protein 2 (Bglap2), catalase, Gp91phox, p22phox and klotho in renal medulla and cortex were analyzed by real-time reverse transcription-polymerase chain reaction.Gene expressions of p22phox and Gp91phox mRNA were increased in the renal medulla of OLETF rats compared with LETO rats. BMP2, cbfa1, MGP and Alp mRNA expression were increased in the renal cortex of OLETF compared with LETO rats. klotho mRNA in the renal medulla of OLETF rats was reduced compared with LETO rats. Catalase mRNA expression of both renal medulla and cortex did not differ between OLETF and LETO rats.These altered gene expression may contribute to rightward shift of pressure-natriuresis and enhancing cardiovascular calcification in diabetic hypertensive rats.