An Experimental Study on Development of Cultured Epithelial Grafting Sheets with Amnion and Oral Epithelial Cells

本研究旨在探讨人口腔黏膜上皮细胞在超干羊膜上作为培养复层上皮片的新来源的可能性。口腔角质形成细胞和超干燥羊膜复合体具有与天然口腔角化粘膜相似的层状上皮层。免疫组织化学显示，口腔角质形成细胞和超干燥羊膜复合体比天然角化粘膜处于更强的增殖状态。这些结果表明，口腔角质形成细胞和超干燥羊膜复合材料可能是利用人类细胞株开发同种异体复层上皮的一种有前途的细胞来源。

The aim of this study was to assess the possibility of human oral mucosal epithelial cells on hyper-dry-amnion can be used as new source for culturing stratified epithelium sheets. The oral keratinocyte and hyper-dry-amnion composites had a well-stratified epithelial layer similar to native oral keratinized mucosa. Immunohistochemistry, the oral keratinocyte and hyper-dry-amnion composites appeared to be in a more proliferative state than native keratinized mucosa. These results suggested that the oral keratinocyte and hyper-dry-amnion composites may be a promising cell source for the development of stratified epithelium allograft sheets using a human cell strain.