A biocomposite material coordinated with a biopolymer hydrogel and mesenchymal stem cells for bone repair

骨整合种植体的生物力学稳定性尤为重要，尤其是种植体表面与骨组织界面的结构操纵所获得的稳定性。通过在生理盐水中放电的方法制备了纳米磷酸三钙固定钛。与羟基磷灰石相比，它通过成骨细胞与b-磷酸三钙之间的协同作用，在表面形成了类似骨的化学结构。本研究通过细胞培养，研究了两种纳米钙磷酸盐固定钛表面形成的矿化层的纳米结构和生物力学性能的差异。扫描探针显微镜观察发现，细胞培养1周后，β-磷酸三钙表面形成的矿化组织粗糙度明显高于羟基磷灰石。对体外生成的多层结构的纳米压痕微观机械评估显示，β-磷酸三钙样品上有更厚的骨样矿化层。通过成骨细胞和纳米β-磷酸三钙之间的协同作用，有望成功地对钛植入物进行修饰。

The biomechanical stability of osseointegrated implants is of particular importance, especially the stability which is achieved from structural manipulation at the interface between the implant surface and the bone tissues. Nanoscale b-tricalcium phosphate-immobilized titanium was prepared by discharge into a physiological buffered saline solution. Compared with hydroxyapatite, it has been shown to be effective in generating a bone-like chemical structure on the surface by cooperative interaction between osteoblastic cells and the b-tricalcium phosphate. The present study, after cell cultivation, investigates the nanostructures and biomechanical property differences of a mineralized layer formed on two samples of nano-calcium phosphate-immobilized titanium. A scanning probe microscope study revealed that the mineralized tissue formed on the b-tricalcium phosphate samples after 1 week of cell culture showed significantly higher roughness, compared with hydroxyapatite samples. Nanoindentation micromechanical evaluation of the in vitro generated multilayered structures exhibited thicker bone-like mineralized layers on the β-tricalcium phosphate samples. A successful modification of titanium implants through the cooperative interaction between osteoblastic cells and nano β-tricalcium phosphate is anticipated.