Effect of amelogenin on angiogenesis in vitro

我们研究了釉原蛋白是否能在体外诱导血管生成。划痕实验和Boiden小室实验表明，釉原蛋白可诱导小鼠内皮细胞系TKD 2细胞迁移。釉原蛋白不能诱导内皮细胞增殖和发育。用引物芯片检测细胞外基质的基因表达，然而，当细胞用釉原蛋白处理时，基因表达没有差异。

We investigated whether amelogenin could induce angiogenesis in vitro. Scratch assay and boiden chamber assay showed that the migration of TKD2 cells, mouse endothelial cell line, was induced by treatment with amelogenin. Cell proliferation and development of endoyhelial cell was not induced by treatment with amelogenin. Gene expression of extracellular matrix was examined by using primer array, however, there is no difference in gene expression when cells were treated with amelogenin.