Cell migration driven self-assembly of microstructure and its application for intracellular nano interface

我们演示了细胞内纳米针插入单个活细胞，这是由细胞驱动的力引起的。细胞驱动的微纳米混合组件组装为纳米生物工程研究提供了一种无需直接机械操作的新型生物微机电系统（bio-MEMS）制造工具的潜在途径。我们描述了一个直径为190nm的单导电钨纳米针的电池驱动自插入。在每个15μm宽的玻璃微孔底部制作单个纳米针。成肌细胞C2C12细胞迁移到微孔中；在3小时内，使用三维激光扫描共聚焦显微镜观察纳米针插入细胞的情况。荧光标记的活细胞观察表明，纳米针插入过程中微孔中没有死亡细胞。细胞内纳米针最多在2天后断裂。

We demonstrated the insertion of an intracellular nanoneedle into a single living cell which was caused by a cell-driven force. The cell-driven assembly of bio-hybrid micro and nanocomponents offers a potential route in nano-bioengineering research on new fabrication tools of bio-micro electro-mechanical systems (bio-MEMS) without direct mechanical manipulation. We describe the cell-driven self-insertion of a single conductive tungsten nanoneedle of 190nm diameter. A single nanoneedle was fabricated on the bottom of each 15μm wide glass microwell. A myoblast C2C12 cell migrated into a microwell; within 3hours, the insertion of a nanoneedle into the cell was observed using a three-dimensional laser scanning confocal microscope. Observation of fluorescence labeled viable cells indicated that no dead cells were present in the microwells during the nanoneedle insertion process. The intracellular nanoneedles were broken after a maximum of 2days.