Electron-beam induced single molecular manipulation and nano processing of living cell in aqueous solution

我们的研究报告了一种在非真空液体环境中对完整的活细胞进行原位EB光刻的新方法;在未来，这项研究可能会导致通过分子力学对细胞膜进行纳米手术。我们期望这一原位纳米加工和细胞纳米刺激的首次演示可以用于膜蛋白动力学变化的真实的时间分析。我们使用EB诱导反应对培养的活细胞进行纳米处理，可以直接操纵环境的分子物理动力学功能和化学性质。而且理论上EB的波长还不到纳米级，在水中的电子射程也是10 s nm。因此，这种方法可以提供新的方法，在原位分子水平上的动力学操纵膜动力学相关的疾病，例如癌症转移，并修复和再生神经元损伤后，通过结合单分子成像，它可以用来了解时空动力学如何影响其机制。

Our study reports a new method of in situ EB lithography in a non-vacuum liquid environment for a complete living cell; in the future, this research could lead to nano-surgery by molecular mechanics on a cell membrane. We expect this first demonstration of in situ nano processing and cellular nano stimulation can be utilized for real time analysis of changes in membrane protein dynamics. Our nano processing on a culturing living cell using an EB-induced reaction could directly manipulate the molecular physical dynamic function and chemical property of the environment. Moreover, theoretically, the EB had less than a nanometer scale wavelength, and the electron range in water was also 10s nm. Therefore this method can provide new approaches for in situ molecular level dynamics manipulation on membrane dynamics-related disorders, for example cancer metastasis, and repairing and regenerating neurons after injury; by combining it with single molecule imaging it can be used to understand how spatio-temporal dynamics affects their mechanisms.