Development of artificial peripheral nerve for regenerative medicine

利用由三个挤出头组成的纺丝系统，成功地制备了不同外径(10 0~80 0μm)和不同内径(内径/外径=0.0 6~0.80)的海藻酸钙中空超细纤维。拉伸导致微纤维的外径减小(小于100μm)。将明胶固定在微纤维中可以增强封闭的哺乳动物细胞，如神经细胞和神经胶质细胞系的黏附和增殖。

Calcium-alginate hollow microfibers with various outer (100-800μm) and inner diameters (inner diameter/outer diameter=0.06-0.80) were successfully fabricated using a spinning system consisted of a triple extrusion head. Stretching the microfibers resulted in decrease in outer diameter of them (less than 100μm). Immobilization of gelatin into the microfibers enhanced adhesion and proliferation of enclosed mammalian cells, such as neuronal cell and glial cell lines.