Screening of excreted proteins of Aspergillus fumigates for clinical application.

我们发现了一些新的真菌蛋白，它们天然存在于细胞表面或通过细胞膜排泄出来。根据转录水平选择候选蛋白进行进一步的基因克隆和抗体产生实验，证实在培养上清液中可以检测到一些蛋白。这表明，这些候选真菌，或者至少其中的一部分，是从真菌细胞分泌到培养基中的。我们开发了一种初步诊断试剂盒，作为研究的应用。

We found some novel fungal proteins which natively exist on cell surface or are excreted out through cell membrane. Candidate proteins were chosen depending on levels of transcription for further experiments of gene cloning and of producing antibody, then confirmed that some proteins could be detected in culture medium. That showed those candidate, or at least a part of those, are excreted out to medium from fungal cell. We developed a preliminary diagnostic kit as an application of the study.