The analysis of the disease-causing gene of the tubercle bacillus and fundamental examination of tuberculosis attack control of the candidate new vaccine strain

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The tuberculosis attracts attention as reemerging infectious disease in late year. 60% of the tuberculosis patient are senior citizens in Japan. The most them are past infection patients. and it is thought as follows, and, as for becoming sick through a life among tuberculosis infection patients, it is thought that a tuberculosis patient equal to or less than 10% has tuberculosis for a life. The macrophage is important as host defense mechanism of the tuberculosis infection. There is NO (nitric oxide) and RNI (reactive nitrogen intermediates) in one of the defense mechanism of a host against tuberculosis. We got the research finding that a ctp F gene may participate in a tubercle bacillus greatly as genetic mechanism of the escape mechanism from this defense mechanism. Our object of this research is the confirmation of this result and elucidation of a concrete function of these genes. We knocked out of ctp F gene by allelic exchange method to make gene deficit strain. We examine influence of the knock out strain revealed by NO and RNI in vitro and compare it with a wild strains. In addition, we let these knock outs strain infect with human THP-1 macrophages and compare knock out strain with wild strains and examine the change of the number of viable bacteria, the viable or death of the cell, and an apoptosis instruction over time. In the future, we perform in vivo experiments with mice.