Reproduction islet transplantation for youth onset I type diabetes The use of the tissue engineering

培养的大鼠胰岛细胞在温度反应培养皿上达到融合后，通过降低培养温度收获成均匀连接的组织片。组织学检查表明，收获的细胞片具有单层二维结构，建立并保持了细胞间的结构连接。免疫组织染色显示胰岛细胞片由胰岛素和胰高血糖素染色阳性细胞组成。通过将胰岛细胞片移植到小鼠皮下，成功地构建并维持了胰岛组织。胰岛生物工程方法的治疗效果也在诱导糖尿病小鼠的移植研究中得到证实。

After reaching confluency on temperature-responsive culture dishes, cultured rat islet cells were harvested as a uniformly connected tissue sheet by lowering culture temperature. Histological examination showed that harvested cell sheet had a monolayered 2-D structure, which established and retained its structural cell-to-cell connections. Immunohistological staining revealed that islet cell sheet comprised of insulin- and glucagon- staining positive cells. By transplanting islet cell sheets into the subcutaneous space of mice, islet tissues were successfully engineered and sustained. Therapeutic effectiveness of this islet bioengineering procedure was also confirmed in the transplantation study to induced diabetic mice.