Investigation of Molecular Mechanisms of Apoptosis using gene knock-out leukemic cell lines

为了探讨糖皮质激素（GC）诱导白血病细胞凋亡的分子机制，我们建立了RCAN 1基因敲除的Nalm 6细胞。RCAN 1是GC的靶基因之一，Nalm 6是在GC处理后经历凋亡的人白血病细胞系。与野生型细胞相比，RCAN 1-/-细胞对GC具有显著的抗性。相反，RCAN 1的过表达导致GC引起的凋亡增强。这些结果表明RCAN 1是GC诱导白血病细胞凋亡的介导因子。

In order to investigate the molecular mechanism of how glucocorticoid (GC) induces apoptosis in certain leukemic cells, we established RCAN1 gene knock-out Nalm6 cells. RCAN1 is one of the target genes of GC and Nalm6 is a human leukemic cell line that undergoes apoptosis upon the treatment with GC. RCAN1 -/- cells were markedly resistant to GC compared with wild-type cells. On the contrary, overexpression of RCAN1 resulted in enhanced apoptosis by GC. These resultsdemonstrate that RCAN1 is a mediator for GC-induced apoptosis in leukemic cells.