Dynamic analysis of physiological barrier function of nasal mucosal epithelia

鼻上皮屏障功能的调节似乎参与了变应性鼻炎的发病机制。经皮失水的测量已被证明是评估皮肤作为保护屏障效率的一种重要的非侵入性方法。虽然鼻黏膜上皮与皮肤一样具有这种保护功能，但其确切机制尚不清楚。我们使用我们的原始适配器检测了人鼻经上皮失水（TEWL），并测量了鼻黏膜的电位差（PD）。我们观察到，局部应用氢水而不是抗坏血酸后，鼻子的TEWL减少。我们也可以检查人咽粘膜后壁的PD。施用HCl后，PD较基态降低。施用ZnCl2后，PD较基础状态显著升高。我们可以建立一种新的模型来评价多酚对鼻黏膜上皮电屏障功能的影响。我们在这项研究中表明，我们的原始模型可能对分析剪切应力对气道上皮的影响非常有用。该模型所获得的数据将有助于了解病理性剪切应力破坏上气道屏障功能的发病机制和治疗方法。我们评估了多阳离子和多阴离子对豚鼠鼻腔和气管上皮细胞经皮电阻（TER）的短期影响，并确定了引起TER改变的途径（经细胞或细胞旁）。原代培养的鼻上皮细胞经多阳离子化后TER的减少可能通过包括离子通道在内的细胞外途径以及紧密连接等细胞外途径引起。此外，肝素等多阴离子在LPS和poly （I:C）作用后可能恢复气道黏膜上皮的屏障功能。少

The regulation of barrier function of nasal epithelium seems to be involved in the pathogenesis of allergic rhinitis. The measurement of transepidermal water loss has been proved to be an important non-invasive method for assessing the efficiency of the skin as a protective barrier. Although the nasal mucosal epithelium has such protective function as well as skin, the precise mechanism is still obscure. We examined the human nasal transepithelial water loss (TEWL) using our original adapter as well as the measurement of electrical potential differences (PD) of nasal mucosa. We observed that TEWL of the nose was decreased after topical application of hydrogen water rather than ascorbic acid.We could also examine PD of the posterior wall of the human pharyngeal mucosa. After application of HCl, PD was decreased compared with the basal state. After application of ZnCl2, PD was significantly increased compared with basal state.We could develop a new model to evaluate the effect of sheer s … More tress on the electrical barrier function of nasal mucosal epithelium. We have shown in this study that our original model might be very useful for analyzing the effect of shear stress on the airway epithelium. The data obtained from this model would contribute to understand the pathogenesis and therapy of the upper airway diseases with the rupture of the barrier function by pathological shear stresses.We have evaluated the short term effect of polycations and polyanions on the transepithelial electrical resistance (TER) of air-interfaced cultured nasal and tracheal epithelial cells excised from guinea pig and to determine the route (transcellualr or paracellular ) that cause the alteration of TER. The reduction in TER of the primary cultured nasal epithelial cells by polycations might be caused via the transcellualr route including ion channels as well as paracellular route like tight junction. Moreover, polyanion like heparin might recover the barrier function of airway mucosal epithelium after application of LPS and poly (I:C). Less