Examination to an amelogenin gene expression in several tissues in a body

本研究对釉原蛋白在小鼠几种组织中的基因表达进行了研究。样本取自胚胎第9天、第18天的小鼠组织Mix、第8周的小鼠组织Mix和几个各靶组织的cDNA。本研究的靶组织取自8周龄小鼠的脑、心、肺、肝、胃、子宫、胎盘、肾脏、胰腺、、肌肉、皮肤、小肠、大肠、脾、卵巢等16个组织。用聚合酶链式反应(PCR)扩增出不同大小的釉原蛋白基因片段(扩增片段为226个碱基)，扩增结果显示，8周小鼠组织Mix和18天小鼠组织Mix基因表达均呈阳性反应。而胚胎9日龄小鼠组织Mix未检测到任何基因的表达。16个组织的PCR扩增结果显示，除胃和胰腺外的14个组织中有釉原蛋白基因的表达。这些结果表明，釉原蛋白基因在各种组织中都有表达，而且这种表达不仅限于发育阶段，在成年小鼠中也有表达。这些结果表明，釉原蛋白可能在体内各组织中起到一定的稳态作用。

The gene expression of amelogenin in several mouse tissues were evaluated in this study. The samples were chosen from embryonic 9day, 18day mouse tissue mix cDNA, 8week mouse tissues mix cDNA and several each target tissues cDNA. The target tissues for this study were selected 16 tissues (brain, heart, lung, liver, stomach, uterus, placenta, kidney, pancreas, testine, muscle, skin, small intestine, large intestine, spleen, and ovary)from 8week old mouse body. The polymerase chain reaction(PCR)was used to amplify several size of amelogenin using a part of exon6 to exon7(small size of PCR amplification : 226bp).The result of PCR amplification showed the positive react of gene expression at 8week mouse tissues mix cDNA and 18day mouse tissue mix cDNA. However none of gene expression was detected by embryonic 9 day mouse tissues mix cDNA. The result of the PCR amplification for 16tissues was obtained amelogenin gene expression in 14 tissues excluded stomach and pancreas. These results suggested that amelogenin genes are expressed in various tissues and this expression is not only restricted to development stage that also expressed in adult mouse. These results suggested that amelogenin might play some homeostatic role in various tissues of the body.