Analysis of cortico-basal ganglia loop circuits with novel retrograde transneuronal tracing technique

采用狂犬病毒逆行跨神经元标记技术，分析了猕猴皮质-基底节区环路的结构基础。我们证明存在一个涉及背纹状体的闭合，平行环路和一个涉及腹侧纹状体的开放环路。此外，我们还克隆了狂犬病毒CVS株的全基因组和所有基因，建立了cDNA质粒狂犬病病毒载体的救援体系。这使我们能够利用制造蛋白如GFP和RFP实现多色逆行跨神经元追踪。

By means of retrograde transneuronal labeling technique with rabies virus, the structural basis for cortico-basal ganglia loop circuits was analyzed in macaque monkeys. We demonstrated the existence of a closed, parallel loop involving the dorsal striatum and an open loop involving the ventral striatum. Moreover, we performed cloning of full-length genome and all genes of the CVS strain of rabies virus, and established the rescue system for a rabies virus vector from cDNA plasmid. This enables us to achieve multi-color retrograde transneuronal tracing with maker proteins such as GFP and RFP.