Recovery and Recycling of cellulase using a functional biopolymer

纤维素酶对纤维素生物质的酶解一直受到人们的关注。本研究提出以木质素基功能性酚醛聚合物“木质素酚”为吸附载体，对纤维素酶进行回收再利用。将纤维素酶简单地固定在木质素酚上，得到不溶于水的纤维素酶-木质素酚复合物。软木对木质素酚的吸附量高于硬木。葡萄糖的存在没有改变吸附量。控制pH和盐浓度对纤维素酶的解吸效果不明显。用有机溶剂沉淀法分离纤维素酶是可行的，但由于纤维素酶对水的溶解度有限，重复利用似乎很困难。纤维素酶-木质素酚配合物可作为固定化纤维素酶，酶活性为天然纤维素酶的20-60%，且最适pH范围较宽。

Enzymatic hydrolysis of cellulosic biomass by cellulase has been receiving attention. In this study, recovery and recycling of cellulase were proposed using a functional lignin-based phenolic polymer "lignophenol" as an adsorption carrier. Cellulase was easily immobilized on lignophenol simply by mixing to give water-insoluble cellulase-lignophenol complex. Adsorption capacity of lignophenol from softwood was higher than that from hardwood. The presence of glucose did not change the adsorption capacity. Control of pH and salt concentration did not work effectively for desorption of the cellulase. Precipitation separation of cellulase with organic solvents was possible, but the reuse seemed difficult because of limited solubility to water. Cellulase-lignophenol complex was available as an immobilized cellulase with 20-60% of enzymatic activity of native cellulase with wider optimum pH range.