Measurement of chemical reaction and structural change of a protein molecule by means of localized surface plasmon resonance scattering spectroscopy.

利用局域表面等离子体共振（LSPR）散射光谱技术测量蛋白质-蛋白质相互作用。建立了一种由流动池、泵浦、全内反射照明和光检测系统组成的装置。本研究还制备了金纳米颗粒沉积的传感器芯片，并将其用于IgG和抗IgG分子之间的免疫亲和性测量，同时研究了一种基于金纳米颗粒等离子体耦合的蛋白质分子结构变化的观测方法。为此，在玻璃基底表面上制备与蛋白质缀合的金纳米颗粒二聚体，并监测共振光散射强度。结果表明，ATP存在时，热休克蛋白70的结构变化率增加。

A protein-protein interaction measurement technique using localized surface plasmon resonance (LSPR) scattering spectroscopy was exploited. An apparatus which consists of a flowcell, pump, total-internal reflection illumination and light detection system was constructed. A gold nanoparticle deposited sensor chip was also fabricated and used for immuno-affinity measurements between IgG and anti-IgG molecule.An observation method for structural change of protein molecules based on plasmon coupling of gold nanoparticle was also investigated in this study. For this purpose, gold nanoparticle dimer conjugated with a protein was fabricated on glass substrate surface, and resonance light-scattering intensity was monitored. It was found that structural change rate of Heat shock protein 70 was increased in the presence of ATP.