Direct protein analysis from electrophoresis gel using a mid-infrared laser

在这项研究中，我进行了蛋白质分析，从丙烯酰胺凝胶电泳后，使用中红外可调谐激光。我报道了IR-MALDI过程受羧基周围的氢键或残余水的影响。丙烯酰胺溶液在6.0μm附近有一个强的吸收带，对应于>C=O伸缩振动模式。用波长为5.8-5.9μm的红外激光照射丙烯酰胺凝胶，获得了胰岛素离子化的质谱图。

In this study, I performed protein analysis from acrlamide gel after electrophoresis using a Mid-infrared tunable laser. I reported that the IR-MALDI process is influenced by the hydrogen bond around the carboxyl group or residual water. The aclylamide solution has a strong absorption bands at around 6.0μm correstponding to the >C=O stretching vibration mode. I obtained the mass spectrum of insulin ionized from acrylamide gel when the sample was irradiated by the infrared laser in a wavelength range of 5.8-5.9μm.