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Experimental design of ELISA for quantitative MCT4 analysis and an investigation of signal transduction cascades

Experimental design of ELISA for quantitative MCT4 analysis and an investigation of signal transduction cascades
用于定量 MCT4 分析的 ELISA 实验设计和信号转导级联研究
批准号:
21500690
负责人:
IIZUKA Kenji
金额:
$2.91万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2009
资助国家:
日本
项目状态:
已结题
起止时间:
2009 至 2011

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中文摘要
翻译
制备了识别MCT4 c末端的抗单羧酸转运蛋白(MCT) 4多克隆抗体和以MCT4分子中间部分合成肽为抗原制备的抗MCT4多克隆抗体。利用这些抗体,我们构建了单个和三明治酶联免疫吸附测定系统,用于血清和/或培养基中MCT4浓度的定量。
英文摘要
Two types of antibodies, anti-monocarboxylate transporter(MCT) 4 polyclonal antibody recognizes C-terminal end of MCT4 and the anti-MCT4 polyclonal antibody created by considering synthetic peptide of the intermediate part of MCT4 molecule as an antigen, were prepared. Using these antibodies, we constructed both a single and a sandwiche enzyme-linked immunosorbent assay system for quantification of MCT4 concentration in serum and/or culture medium.
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DOI: --
发表时间: 2010
期刊:
影响因子: --
作者: [飯塚健治, 森田憲輝, 町田拓自, 平藤雅彦]
通讯作者: 平藤雅彦
An investigation of endocrine factors from cultured skeletal muscle cells and the effect of cell pressurization
  • 批准号:
    24500864
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 资助金额:
    $3.41万
  • 财政年份:
    2012
  • 负责人:
    IIZUKA Kenji
  • 依托单位:
海外基金