A study on new approaches in soft x-ray microscopy of living cells in the spectral region of "carbon window"

提出了在“碳窗”光谱区进行软X射线显微镜观察活细胞的方法。一个新开发的低温样品系统，使用液氮和氧气安装在软X射线显微镜能够进行“碳窗”观察。使用显微镜，减轻辐射损伤的聚苯乙烯乳胶球在低温温度下进行了验证。我们还成功地在低温下对生物标本进行了实验观察。此外，碲对耐辐射奇异球菌的图像对比度增强产生了影响，这是“碳窗”中活细胞观察的主要候选者。

Soft X-ray microscopy in the spectral region of "carbon window" was suggested as a way to observe a living cell. A newly-developed cryogenic sample system with the use of liquid nitrogen and oxygen gas was installed in the soft X-ray microscope capable of performing a "carbon window" observation. Using the microscope, alleviation of radiation damage in polystyrene latex spheres at cryogenic temperatures was verified. We have also successfully made an experimental observation of biospecimens at cryogenic temperature. Furthermore, tellurium produced an effect on an image contrast enhancement for Deinococcus radiodurans which is a major candidate for a living cell observation in "carbon window".