Clarification of the physiological role of diacylglycerol in E. coli

为了阐明甘油二酯在大肠杆菌中的生理作用，列出了10个可能参与甘油二酯生物合成的基因，并构建了多个基因敲除菌株。结果表明，可构建一株生长对冷敏感、甘油二酯含量大大降低的8倍基因敲除菌株。然而，耗尽还没有完全完成。另一方面，我们确定了糖脂MPIase的结构，并分析了参与蛋白质整合到膜中的功能。

To clarify the physiological role of diacylglycerol in E. coli, ～10 genes, which may be involved in diacylglycerol biosynthesis, were listed up, and then constructed multiply knock-out strains. Resultantly, an eightfold knock-out strain could be constructed, of which the growth was cold-sensitive and the diacylglycerol content was greatly reduced. However, the depletion was not complete. On the other hand, we determined the structure and analyzed the function of glycolipid MPIase involved in protein integration into membranes.