Development of a cancer treatment technique using intracellular collapsing liposomes directed to cancer with double safety systems

我们的目标是开发一种新的、有效的、安全的使用具有双重安全系统的细菌毒素治疗癌症的技术。首先，研制了一种具有安全系统和可变靶向能力的靶向毒素。证实用靶向毒素和抗癌胚抗原(CEA)免疫球蛋白制备的脂质体包埋抗癌药物能特异性靶向CEA阳性癌细胞，并能有效延长CEA阳性癌细胞接种小鼠的生命。第二种带有安全系统的细胞毒效应毒素也被生产出来，因此开发一种使用这两种带有安全系统的毒素治疗肿瘤的靶点可变的新技术成为可能。

We aimed to develop a novel effective and safe technique for cancer treatment using bacterial toxins with dual safety systems. First, a targeting toxin with safety system and the variable targeting ability was developed. It was confirmed that the liposomes enclosed anticancer drug prepared by using the targeting toxin and anti-carcinoembryonic antigen(CEA) IgG could specifically target to CEA-positive cancer cells and were effective to prolong life of mice inoculated with CEA-positive cancer cells. The second cytotoxic effector toxin with safety system was also produced, so the development of a novel technique for the target-variable cancer treatment using both toxins with safety systems came in sight.