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Study on the surface antigens of streptococcal cells.

链球菌细胞表面抗原的研究。

基本信息

批准号：
63570873
负责人：
NAGAMUNE Hideaki
金额：
$ 0.26万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

项目摘要

In the present study, the nature of cell surface antigens of streptococci, especially mutatis group streptococci was analyzed using several monoclonal antibodies prepared against 8 serotypes of this group as probes. The following results were obtained. 1. Cri-protein, a 185K dalton cell surface glycoprotein antigen specific for serotype a, was recognized. Its type-specific antigenie determinant seemed to be a mannosamine structure on its sugar chain(s). 2. The serotype b-specific antigen was lipoteichoic acid and its antigenic determinant seemed to be a galactose structure. 3. The serotype c, e, and f-specific antigen was a cell wall polysaccharide and its antigenic deteminant seemed to be a N-acdtylglucosamine structure. 4. The surface protein antigen specific for serotype g was also analyzed. However, a certain strain of this serotype did not possess this antigen. 5. The common antigen between serotypes d, and g and serotype h was a cell wall polysaccharide antigen and its antigenic … More deteminant seemed to be contain rhamnose and beta-linked galactose structures. 6. The common antigen among serotype a, serotype d, and serotype h was also a cell wall polysaccharide and its antigenic determinant seemed to be a galactose alpha 1->6 glucose structure. We have established an effective analytical system of the reactivity between the streptococcal antigen and monoclonal antibody, enzyme immunoassay on microtiter plate wells coated with the streptococcal whole cells, cell wall polysaccharide or glycoprotein antigen. Moreover, we have proven the usefulness of competitive enzyme immunoassay as a universal method for analysis of polysaccharides and have developed a reliable immunological method for species identification of mutatis group streptococci. As mentioned above, the present study revealed substantial information on the nature of cell surface antigens of mutatis group streptococci. However, how these antigens are involved in the caries process still has to be further investigated. Less

本研究用抗变形链球菌8个血清型的单克隆抗体作探针，分析了变形链球菌细胞表面抗原的性质。获得了以下结果。1. Cri蛋白是一种185 K道尔顿的细胞表面糖蛋白抗原，对血清型a具有特异性。其型特异性抗原决定簇似乎是其糖链上的甘露糖胺结构。2.血清b型特异性抗原为脂磷壁酸，其抗原决定簇似为半乳糖结构。3.血清型c、e、f的特异性抗原是一种细胞壁多糖，其抗原决定簇可能是N-乙酰氨基葡萄糖结构。4.还分析了对血清型g特异的表面蛋白抗原。然而，该血清型的某些菌株不具有该抗原。5. D、G血清型与H血清型的共同抗原为细胞壁多糖抗原，其抗原性与 ...更多信息确定物似乎含有鼠李糖和β-连接的半乳糖结构。6.血清型a、血清型d和血清型h之间的共同抗原也是细胞壁多糖，其抗原决定簇似乎是半乳糖α 1->6葡萄糖结构。我们建立了一种有效的链球菌抗原与单克隆抗体反应的分析体系，即用链球菌全细胞、细胞壁多糖或糖蛋白抗原包被的微孔板威尔斯酶免疫分析法。此外，我们已经证明了竞争性酶免疫分析作为一种通用的方法，用于分析多糖的有用性，并已开发出一种可靠的免疫学方法的mutatis组链球菌的物种鉴定。如上所述，本研究揭示了变形链球菌细胞表面抗原性质的大量信息。然而，这些抗原如何参与龋齿过程仍有待进一步研究。少