Roles of transcription factors during endothelial-mesenchymal transformation in the developing chick heart

我用鸡胚心脏研究了sox9在缓冲组织形成过程中内皮-间质转化（EMT）中的作用。首先，我们通过原位杂交检测了它们在发育中的心脏中的表达模式。Sox9在流出道和房室管区活化的内皮细胞和间充质细胞中表达。第二，在体外实验中，sox9 siRNA抑制内皮转化。转染sox9的内皮细胞出现细胞迁移，但未侵入凝胶晶格。而转染sox9和slug的内皮细胞转化为间充质细胞。综上所述，结果表明sox9和slug可能在心内膜上皮-间质转化过程中发挥重要作用

I examined role of sox9 in the endothelial-mesenchymal transformation(EMT) during cushion tissue formation using chick embryonic hearts. First, we examined their expression patterns in the developing heart by in situ hybridization. sox9 was expressed in the activated-endothelial and mesenchymal cells of the outflow tract and the atrioventricular canal regions. Second, sox9 siRNA inhibited endothelial transformation in vitro assay. Endothelial cells transfected with sox9 showed cell migration, but did not invade into the gel lattice. However endothelial cells transfected with sox9 and slug transformed to mesenchymal cells. Taken together, results suggest that sox9 and slug may play an important role during endocardial epithelial-mesenchymal transition