The effects to damaged joints by using hyaluronic acid with various molecular weight

我们检测了损伤关节组织中透明质酸合成酶（HASs）和透明质酸酶（Hyals）的表达及其相互关系。我们还研究了低分子量透明质酸（MW）在类风湿性关节炎（RA）患者滑液中的细胞毒性。在RA的炎症活动期，滑膜组织中的炎症细胞产生HA -3，细胞可合成低分子量的HA。在RA炎症活动期，透明质酸酶(Hyal)-1和Hyal-2阳性细胞数量增加，HA可能退化至低MW。在一些RA病例中，滑液中有高活性的Hyal。低MW HA可引起滑液润滑功能障碍，并具有促炎作用，因此维持HAS1、has2、has3或Hyals之间的平衡可能对维持关节功能正常很重要。

We examined the expression of hyaluronic acid(HA) synthases(HASs) and Hyaluronidases(Hyals) in damaged articular tissues, and the relationship between them. We also examined the cytotoxicity of HA with low molecular weight(MW) in the synovial fluid of patients with rheumatoid arthritis(RA). In active inflammatory stage of RA, inflammatory cells in synovial tissue produce HAS-3 and the cells may synthesize HA with low MW. In active inflammatory stage of RA, the number of positive cells of hyaluronidase(Hyal)-1 and Hyal-2 increased and HA may be degenerated to lower MW. In some RA cases have high activity of Hyal in synovial fluid. HA with low MW may cause dysfunction of synovial fluid as lubricant and it also has proinflammatory effects, so the balance between HAS1, 2, 3 or Hyals may be important to maintain the joint's function normal.