A quantitative analysis of bacterial flora in the small intestine

由于取样困难，小肠内细菌菌群的状态尚不清楚。采用双球囊内窥镜取肠液进行细菌培养定量研究。在胃和十二指肠中发现少量细菌，而在下空肠和后空肠中发现大量细菌。在空肠下部，厌氧条件建立。在大约60%的健康成人中检测到艰难梭菌。半数分离菌株具有肠毒素基因（toxA和toxB）。这种细菌是在回肠上和回肠后分离出来的。在感染幽门螺杆菌的成人中，细菌可以用从胃到空肠的汁液培养。

The state of bacterial flora in the small intestine is unclear because of the difficulty for taking samples. We investigated bacteria quantitatively by cultures from small intestinal juices which were taken with a double-balloon endoscopeas a new technique. A few of bacteria were found in the stomach and duodenum, while we detected many bacteria on and after the lower jejunum. In the lower jejunum, anaerobic condition is established. Clostridium difficile was detected from approximately 60% of healthy adults. A half of isolated strains had enterotoxin genes(toxA and toxB). This bacterium was isolated on and after the ileum. In adults infected with Helicobacter pylori, the bacterium was culturable with juices from the stomach to the jejunum.