Studies on the early cancer detection with urinary diacetylspermine and its application

N^1, N^<12>-二乙酰精胺（DiAcSpm）在癌症患者早期尿中经常升高，因此被认为是有希望的肿瘤标志物。在本研究中，我们首先详细分析了健康人尿中DiAcSpm水平，并建立了临界值来区分正常和异常的DiAcSpm排泄水平。然后，我们开发了一种简单快速测定DiAcSpm的免疫层析装置。这种简单的检测方法的推广可能有助于增加早期发现癌症患者的机会。我们进一步的分析表明，从癌症发展的早期开始，DiAcSpm在癌症组织中就增加了。这一发现一方面为我们提供了一种早期癌症检测的新方法，也为回答为什么早期癌症患者尿液中DiAcSpm增加的问题提供了有用的线索。

N^1, N^<12>-diacetylspermine(DiAcSpm) in urine is frequently increased in cancer patients at earlier stages of the disease and hence is considered as promising tumor marker. In the present study we first analyzed the urinary DiAcSpm level of healthy persons in detail, and established cut-off values to distinguish between normal and abnormal levels of DiAcSpm excretion. We then developed an immunochromatographic device for simple and rapid DiAcSpm determination. The spreading of this simple determination procedure may help in increasing the opportunity of early detection of cancer patients. Our further analysis revealed that DiAcSpm is increased in cancer tissues from very early stages of cancer development. This finding provides us with a new method for early cancer detection on one hand, and a useful clue to answer the question of why DiAcSpm is increased in the urine from early cancer patients.