Generation of bone marrow failure model mice by autoantibodies inducing cytokine production

Moesin KO小鼠生长正常。moesin KO小鼠的体重明显低于WT小鼠。moesin KO小鼠全血细胞计数显示白细胞减少和大细胞性贫血；血小板计数具有可比性。Moesin KO小鼠脾脏较WT小鼠重，脾脏卵泡形成模糊，CD3^+淋巴细胞增加，CD19^+淋巴细胞减少。moesin KO小鼠的BM呈正常细胞性，脾脏中CD3^+淋巴细胞增加，CD19^+淋巴细胞减少。moesin KO BM中谱系阴性、Sca-1^+和c-Kit^+(LSK)小鼠hsc的数量倾向于低于WT小鼠。将能够产生抗Moesin抗体的Moesin KO脾脏/BM细胞注入WT B6雄性小鼠BM。与移植WT脾/BM细胞的WT B6雄性小鼠相比，移植小鼠血浆中白细胞减少，tnf α和ifn γ升高，但未见贫血和血小板减少。

Moesin KO mice grew normally. However, the body weight of moesin KO mice was lower than that of WT mice. Complete blood counts of moesin KO mice revealed leukocytopenia and macrocytic anemia ; platelet counts were comparable. Moesin KO mouse spleens were heavier than those of WT mice and follicle formation in spleens of moesin KO mice were obscure with increased CD3^+lymphocytes and reduced CD19^+lymphocytes as compared with WT mice. BM of moesin KO mice was normocellular and characterized by increased CD3^+lymphocytes and reduced CD19^+lymphocytes as seen in spleens of moesin KO mice. The number of lineage negative, Sca-1^+, and c-Kit^+(LSK) mouse HSCs in moesin KO BM tended to be lower than that of WT mice. Moesin KO spleen/BM cells capable of producing anti-moesin antibodies were infused into BM of WT B6 male mice. Transplanted mice showed leukocytopenia and increase of TNFαand IFNγin plasma compared to WT B6 male mice transplanted with WT spleen/BM cells, but anemia and thrombocytopenia were not observed.