Involvement of aquaporin-3 in keratinocyte proliferation and tumorigenesis

我们研究了水通道蛋白-3(AQP3)在特应性皮炎(AD)等皮肤病的表皮增生中的作用。我们发现在人类AD皮损的表皮中AQP3的转录和蛋白表达显著增加。人AQP3 DNA载体可上调角质形成细胞中AQP3的表达，促进细胞增殖。在小鼠AD模型中，AQP3在野生型小鼠的表皮中强烈过表达。AQP3基因缺陷小鼠表皮增生减轻，角质形成细胞数量减少，屏障功能正常。这些结果提示，AQP3通过上调AQP3的表达进而促进角质形成细胞的增殖参与了表皮增生。

We investigated the involvement of aquaporin-3(AQP3) in epidermalhyperplasia in skin diseases, such as atopic dermatitis(AD). We found significantly increasedAQP3 transcript and protein expression in the epidermis of human AD lesions. The upregulation ofAQP3 expression in human keratinocytes by transfection with human AQP3 DNA plasmid increased cell proliferation. In mouse AD models, AQP3 was strongly overexpressed in the epidermis in wild-type mice. Epidermal hyperplasia was reduced in AQP3-deficient mice, with a decreased number of proliferating keratinocytes and normal barrier function. These results suggest the involvement of AQP3 in epidermal hyperplasia by a mechanism involving upregulated AQP3 expression and consequent enhancement of keratinocyte proliferation.