A prophylaxis and a treatment for radiation induced mucosal damage-Efficacy of zinc to radiation proctitis using an animal experimental model-

选用雌性Wistar大鼠54只。所有大鼠在戊巴比妥钠麻醉下接受单次25Gy射线照射，不进行任何侵入性操作。将波拉普锌制成软膏。照射后每天直肠给药，不作任何麻醉。照射后第10天处死动物。处死后立即行结肠镜检查以评估肉眼所见。在结肠镜检查后，切除直肠进行病理评估。病理评估评估黏膜的形态损害、炎症程度和炎症深度。结果从0到4进行分级，并根据适用的较轻或较严重的情况进行比较。在所有标准中，PZ(+)组的粘膜损伤倾向于比其他组轻。此外，PZ(+)组与对照组在内窥镜检查结果、炎症程度和炎症深度方面也有显著差异。该模型是一种实用的放射性直肠炎大鼠模型。本研究通过大鼠模型验证了波拉普锌对急性放射性直肠疾病的疗效。

Fifty-four female Wistar rats were used. All rats were irradiated with a single fraction of 25 Gy without any invasive procedures under pentobarbital anesthesia. Polaprezinc was prepared as ointment. Ointment was administrated rectally without any anesthesia each day after irradiation. All animals were sacrificed on the tenth day following irradiation. Colonoscopy was performed to evaluate the macroscopic findings immediately after sacrifice. The rectum was excised for a pathological evaluation following colonoscopy. The pathological evaluation assessed the morphological mucosal damage, the degree of inflammation, and the depth of inflammation. The results were graded from 0 to 4, and compared according to milder or more severe status as applicable. The PZ(+) group tended to show milder mucosal damage than the other groups in all criteria. In addition, significant differences were observed between the PZ(+) group and the Control group in the endoscopic findings, the degree of inflammation and the depth of inflammation. This model was confirmed to be a useful experimental rat model for radiation proctitis. The present study demonstrated the efficacy of Polaprezinc against acute radiation-induced rectaldisorders using the rat model.