Systematic strategy for non-invasive neuro-reconstruction

本研究证明重复经颅磁刺激(RTMS)可诱导对致死性脑缺血后海马神经元迟发性神经元死亡(DND)的缺血耐受。双侧颈总动脉阻塞(BCCO)。在短暂性脑缺血发作后7天定量评估海马区CA1区迟发性神经元死亡的程度。我们评估了rTMS在BCCO前2-5天交付时，迟发性神经元死亡是否会像在缺血耐受中所报道的那样显著减轻。在BCCO前3天、4天或5天给予刺激时，也会看到类似程度的神经元保留。以25和50赫兹的频率进行刺激，评价rTMS的有效性。

The present study demonstrates that repetitive transcranial magnetic stimulation(rTMS) induces ischemic tolerance against delayed neuronal death(DND) of hippocampal neurons following an otherwise lethal ischemic insult.Various regimens of rTMS were delivered to adult gerbils at various times prior to an episode of ischemia induced by transient(5min.) bilateral common carotid artery occlusion(BCCO). The extent of delayed neuronal death in the CA1 region of the hippocampus was assessed quantitatively 7 days after the transient ischemic episode.We evaluated whether when rTMS delivered 2-5 days prior to BCCO, delayed neuronal death will be substantially attenuated as reported in ischemic tolerance. A similar degree of neuron sparing will be seen when stimulation was delivered 3, 4, or 5 days prior to BCCO. Effectiveness of rTMS was evaluated whether when stimulation was delivered at frequencies of 25 and 50 Hz.