Establishment of a Novel Therapeutic Strategy Using Tissue Regeneration for Intractable Spinal Infections.

利用超声溶解沉淀法在饱和酸性溶液中部分溶解商业多孔生物陶瓷，并在其表面析出磷酸钙微晶。通过控制骨形态发生蛋白-2的吸附和释放特性，制备出具有良好生物吸附和成骨诱导作用的磷酸钙陶瓷。作为生物陶瓷的一种表面改性技术，建立了商品化的肠外液超声处理技术，阐明了抗生素(头孢唑林)在陶瓷表面的吸附与释放特性。合成了适合于组织再生的磷灰石与胶原的仿生复合材料，并对其良好的生物吸收性能进行了研究。在对顽固性脊柱感染患者进行定量评估方面，应用FDG-PET CT建立了8例顽固性脊柱感染患者感染部位的严重程度图。

Commercial porous bioceramics were partially dissolved in saturated acid solutions and calcium phosphate microcrystals were precipitated on the surfaces of bioceramics by the supersonic dissolution -precipitation method. Calcium phosphate ceramics having excellent bio-absorption and osteoinduction were newly produced by controlling adsorption and release characteristics of bone-morphogenetic protein (rhBMP-2). As a surface modification technique of bioceramics, the supersonic treatment technology using commercial parenteral fluids was established and antibiotic (Cefazolin)-adsorption and release characteristics on the ceramics were clarified. Biomimetic composites of apatite and collagen which were suitable for tissue regeneration were synthesized and the good bio-absorption was recognized. Concerning a quantitative evaluation for patients with intractable spinal infections, severity maps of infection sites were created in 8 cases of intractable spinal infections by using FDG-PET CT.