Study of proper mechanism of repair and regeneration after kidney injury evoked by novel gammalactone low molecular compounds derivatives with cytokines

采用单侧输尿管完全梗阻14d，松解后观察6d的动物模型，可明显观察到乳头底部直内翻降束交界处周围的细胞浸润和聚集，并在凹陷的盆腔尿路上皮层下扩散。这些组织反应的病理损伤暂定为损伤小管修复或再生的“壁龛”。为了探索“利基”，重组大鼠肿瘤坏死因子-α的有益治疗诱导了一些适当的基质反应，主要是围绕小管周围间隙的1型胶原。相反，用新的苯并异呋喃酮治疗在Galectin-3或rBAT阳性的细胞聚集的“壁龛”上诱导了另一种适当的基质反应，具有血管生成和抗凋亡作用。目前的研究表明，效力超过10倍的苯并异呋喃类化合物可能是治疗难治性慢性移植肾肾病的候选药物。

Using animal model of unilateral ureteral, complete obstruction for 14 days with for six days observation after release, cellular infiltration and accumulation surrounding crossing points of bundle of descending varsa recta at the base of papillae, and propagating beneath depressed pelvic urothelial layer, was remarkably observed. Those pathologic lesions of tissue responses were tentatively called as "niches" for repair or regeneration of injured tubules. To explore"niches", beneficial treatment with recombinant rat TNF-alpha induced some proper matrix response with dominant type-1 collagen surrounding peritubular space. In contrast, treatment with novel benzoisofuranone induced another proper matrix response at "niches" with galectin-3 or rBAT positive cellular accumulation with angiogenesis and anti-apoptosis. The present studies suggested that ten times more potent benzoisofuranone compounds might be a candidate as promising medicine for intractable chronic renal allograft nephropathy