Cancer stem cell and EGFR target therapy for head and neck cancer

在头颈部癌细胞群中EGFR表达及与MT1-MMP外观相关性的检查中，当EGFR加入HSC4时，承认MT1-MMP外观增加。即对EGFR而言，不仅通过细胞增殖，还通过MT-MMP参与肿瘤的浸润性增殖的可能性，由于与肿瘤干细胞相关的MT1-MMP的相关性已被承认，因此提出了EGFR与肿瘤干细胞的相关性。当检测作为HSC4肿瘤干细胞的克隆形成能力时，26%的细胞具有克隆形成能力，60%的克隆导致内部ALP阳性。证实了Oct3/4和Nanog作为肿瘤干细胞标志物在HSC4中的表达。用流式细胞法从HSC4中鉴定出SP细胞。结果鉴定出0.3-0.4%的SP细胞。在SP细胞中，Oct3/4和Nanog的出现明显增强。更多的癌症干细胞标记物出现在SP细胞和非SP细胞中，用FACS进行分离鉴定。从头颈部癌细胞SAS、SCC4中分离鉴定SP细胞和MP细胞，并观察肿瘤干细胞标记物的形态。Oct3/4和Nanog的出现由于显著的HSC4而上调。本文对41例舌癌综合征早期I/II期患者的肿瘤干细胞标志物外观及淋巴结变化与转移（DNM）的相关性进行了研究。因此，它有意地与DNM、10月3/4日和Nanog出现相关。根据早期舌癌的各种病理特征、肿瘤干细胞标记物的出现和淋巴结分布，通过多因素分析检验其与转移（DNM）的相关性。结果证实肿瘤血管侵及Oct3/4为独立危险因素，向DNM证实其为独立危险因素。对于EGFR，我们认为Oct3/4作为肿瘤干细胞标志物，通过EGFR参与肿瘤的浸润和转移，与肿瘤的复发和转移有着密切的关系。少

In the EGFR expression in the head and neck cancer cell stock and the examination concerning the correlation with the MT1-MMP appearance, when EGFR was added to HSC4, an increase in the MT1-MMP appearance was admitted. That is, the possibility of taking part was suggested in the permeation proliferation of the tumor through not only the cell proliferation but also MT-MMP as for EGFR and because the correlation with MT1-MMP that related to the cancer stem cell had been admitted, the correlation of EGFR and the cancer stem cell was suggested. When the clone formative ability as the cancer stem cell of HSC4 was examined, 26% cells had the clone formative ability and the clone of 60% was cause ALP on the inside positivity. Expression of Oct3/4 and Nanog as cancer stem cell marker in HSC4 was proved. The SP cell was identified from HSC4 by FACS. As a result, SP cell of 0.3-0.4% was identified. The appearance of Oct3/4 and Nanog was accentuation in the SP cell in significant as a result of e … More xamining the various cancer stem cell markers appearance in the SP cell and the non-SP cell that the separation identification is done with FACS. The SP cell and the MP cell were identified from head neck cancer cell SAS, SCC4 apart and the cancer stem cell marker s appearance was examined. The appearance of Oct3/4 and Nanog was up regulated as a result in significant as well as HSC4.Cancer stem cell marker appearance and the lymph node in 41 examples of stage I/II of the early stage of the example of the tongue cancer syndrome, the correlation with metastasis(DNM) was examined. As a result, it intentionally correlated to DNM, Oct3/4, and the Nanog appearance. After various and pathology features, the cancer stem cell marker appearance, and lymph nodes in the early stage tongue cancer, the correlation with metastasis(DNM) was examined by the multivariate analysis. As a result, the vessel invasion of the tumor and Oct3/4 were proven and it was proven to DNM that it was an independent risk factor. As for EGFR, it was suggested that Oct3/4 be deeply related to the recurrence of cancer and the metastasis as the cancer stem cell marker in relation to permeation and the metastasis of cancer through EGFR above. Less