Development of new oral cancer treatment as molecular target for antiapoptosis protein and NF-kB

XIAP是凋亡蛋白家族的一员，通过阻断caspase介导的细胞凋亡与细胞存活相关。XIAP在多种恶性肿瘤中表达。据报道，在各种恶性肿瘤中，XIAP的过表达是一个较差的预后因素。本研究旨在探讨XIAP蛋白在口腔鳞状细胞癌（OSCC）中的表达，并探讨其表达与临床分期、组织学分化及侵袭方式的关系。本研究采用人OSCC细胞系。正常牙龈上皮细胞作为对照。western blot检测培养细胞XIAP、cIAPs及survivin的表达。从85例手术或活检后的OSCC患者中获得组织标本。免疫组化法检测其表达。在所有癌细胞中都检测到这些蛋白的表达，但在正常细胞中没有。85例OSCC的免疫组化分析显示，73例（86%）OSCC表达XIAP。XIAP表达与临床分期、侵袭方式分型无相关性。XIAP表达与组织学分化之间存在显著差异。无染色和弱染色癌多数分化良好。相比之下，在低分化癌中经常发现强烈而广泛的染色。

XIAP is a member of the inhibitor of apoptosis protein family, which is associated with cell survival by blocking caspase-mediated apoptosis. XIAP is expressed in various malignant tumors. The overexpression of XIAP has been reported to be a poorer prognostic factor in various malignancies. present study were to evaluate the expression of XIAP protein in oral squamous cell carcinoma(OSCC) and to elucidate the relationships among the XIAP expression, clinical stages, histological differentiation and classification of invasion mode. human OSCC cell lines were used in this study. Normal gingival epithelial cells served as control. XIAP, cIAPs and survivin expressions of cultured cells wert detected by western blot. Tissue specimens were obtains from 85 patients with OSCC after surgery or biopsy. Their expression was detected by an immunohistochemical method. The expression of those proteins was detected in all cancer cells, but not in normal cells. Immunohistochemical analysis of 85 cases of OSCC showed that 73(86%) cases expressed XIAP. There was no relationship between XIAP expression and clinical stages, or classification of invasion mode. There were significant differences between XIAP expression and histological differentiation. Most of non-staining and weakly staining of cancer was well differentiated. In contrast, intense and extensive staining was frequently found in poorly differentiated cancer.