Comparative evolutionary studies on three-dimensionally positioning of the chromosome territories in the primate iPS cell nuclei

本研究从染色体区域和基因组区域在细胞核内的空间定位角度出发，通过对人和类人猿的比较分析，试图找出诱导性多能干细胞形成的分子机制，以及在灵长类进化过程中出现了哪些人类特有的特征。通过使用Oct 3/4、Sox 2、Klf 4、c-Myc和Nanog的DNA探针及其染色体涂料对人类、黑猩猩、大猩猩和猩猩成纤维细胞核进行3D-FISH技术分析，已经揭示了空间排列的基本特征，但由于缺乏灵长类iPS细胞，尚未进行分析。在积累iPS细胞时，我会进行分析并讨论问题。

In this study from the viewpoint of spatial positioning of chromosome territories and genomic regions within the nucleus I would try to find the molecular mechanism of why the iPS cells are established and to identify what the human specific characteristics have been appeared during the course of primate evolution by comparative analysis in human and great apes. Analysis by 3D-FISH technique using the DNA probes with Oct3/4, Sox2, Klf4, c-Myc, and Nanog, and their chromosomal paints onto human, chimpanzee, gorilla, and orangutan fibroblast cell nuclei, basic characteristics of spatial arrangements have been revealed but analysis has not yet been carried out due to shortage of primate iPS cells. When accumulating iPS cells, I would perform the analyses and discuss the issues.