Investigation and application of new metabolic pathways involved in the synthesis of inosinate and guanylate

研究了参与肌苷和鸟苷合成的新代谢途径。分别以香菇(Lentinula Edodes)、日本养鱼(Trachurus Japan Onicus)和萝卜(Raphanus Sativus)为代表的食用菌、鱼类和蔬菜为研究对象。根据放射性标记前体的原位代谢去向和酶的体外活性，检测了这些食品中嘌呤核苷酸的代谢情况。肌苷和鸟苷在所有食物中都是通过抢救途径合成的。有人认为，在一定的烹饪、加工和保存条件下，抢救途径在这些食品中是起作用的。

New metabolic pathways involved in the synthesis of inosinate and guanylate were investigated. Shiitake mushrooms(Lentinula edodes), cultured Japanese jack mackerel(Trachurus japonicus) and Japanese radish(Raphanus sativus) were chosen as the representatives of mushrooms, fishes and vegetables, respectively. Metabolic profiles of purine ribonucleotides in these foods were examined based on the in situ metabolic fate of radio-labelled precursors and the in vitro activities of enzymes. Inosinate and guanylate were synthesized via salvage pathways in all foods. It was suggested that salvage pathways were operative in these foods under certain conditions of cooking, processing and preservation.