Do the dental factors effect the DNA damage in the mucosal cell?

[前言]微核(MN)试验是检测DNA损伤最敏感的指标之一。锰频率已被用来研究各种化学物质的遗传毒性。在这项研究中，我评估了粘膜细胞微核率与牙科因素(例如牙周炎状况、口腔卫生习惯、牙齿金属和牙齿树脂)之间的关系。我们的目的是研究粘膜细胞MN检测的混杂因素，并建议在粘膜细胞中进行更准确的MN检测。[材料与方法]该研究方案由山形大学医学院伦理委员会批准。研究小组由97名男性组成，他们在工作或休闲时间没有接触过化学物质。[结果]多因素逐步回归分析显示，年龄对胃粘膜细胞微核率有影响。在该模型中，年龄(β=0.45)是唯一显著增加粘膜细胞微核率的危险因素。该模型的校正决定系数(R^2)为0.20。因此，粘膜细胞微核率的变异率有20.0%随年龄增长而扩大。在这个模型中，所有的牙齿因素都没有统计学意义。[讨论]我揭示了牙齿因素不影响粘膜细胞微核率的可能性。进一步的研究需要监测血清或头皮头发中的金属含量，以探讨粘膜细胞中的MN试验与牙科因素的关系。

[Introduction] The micronuclei (MN) assay is one of the most sensitive markers of DNA damage. MN frequency has been used to investigate the genotoxicity of various chemicals. In this study, I evaluated the relationship MN frequency in the mucosal cells and dental factors (ex : periodontitis status, habit of oral hygiene, metals of teeth and resins of teeth,). Our aim was to research the confounding factor for MN test in the mucosal cells and to suggest the more precise MN test in the mucosal cell. [Materials and Method] The study protocol was approved by the Ethics Committee of Yamagata University School of Medicine. The study group consisted of 97 males who had not been exposed to chemicals during their work or leisure time. [Result] The forward stepwise multiple regression analysis showed the effect of age on the MN frequency in the mucosal cells. Age (β=0.45) was only risk factor that significantly increased MN frequency in the mucosal cells in this model. The adjusted coefficient of determination (R^2) in this model was 0.20. Therefor 20.0% of the variability in the MN frequency in the mucosal cells was exppained by age. All of the dental factors in this model were not statistically significant. [Discussion] I revealed the possibility that the dental factors didn't affect the MN frequency in the mucosal cells. Further research is needed with monitoring the metal levels in the serum or scalp hair to investigate the relationship MN test in the mucosal cells and dental factors.