Development of a pyrosequence-based technique for HIV drug-resistance diagnosis

本研究的目的是开发一种基于焦磷酸测序的单细胞分析技术，以准确识别HIV内的多种突变，并对HIV准种形成的相关机制进行基于序列的阐明。结果，我们成功地建立了一个低至10个拷贝的cDNA分子测量系统。此外，我们还成功地追踪了HIV准种相关的原病毒DNA、Gag和PR亚型之间的共同进化。

The aim of this research is to develop a single-cell analytical technique based on pyrosequencing to accurately identify diverse mutations within HIV and to conduct sequence-based elucidation of the mechanisms related to HIV quasispecies formation. As a result, we were successful in the establishment of a system for measuring cDNA molecules to as low as 10 copies. Furthermore, we were also successful in tracing the coevolution between the subtypes of HIV quasispecies-related proviral DNA, Gag and PR.