Characterization of the plastidial pyruvate transporting mechanism

丙酮酸是植物质体内多种代谢途径的必需代谢物，包括C_4光合CO_2-浓缩机制。尽管丙酮酸进入质体的关键作用，介导丙酮酸运输的分子仍然是未知的。利用差异转录组分析的C3和C4植物属黄曲霉，我们已经确定了一种新的质体本地化的转运蛋白基因，命名为BASS 2，胆汁酸：钠同向转运蛋白家族蛋白2，其转录本是丰富的C4植物，具有钠依赖性丙酮酸转运蛋白。重组BASS 2显示钠依赖性丙酮酸摄取活性。

Pyruvate is an essential metabolite for multiple plastid-localized metabolic pathways of plants, including the C4 photosynthetic CO, -concentrating mechanism. Despite the crucial role of pyruvate import into plastids, the molecule mediating pyruvate transport is still unknown. Using differential transcriptome analyses of the C3 and C4 plants of the genus Flaveria, we have identified a novel plastid-localized transporter gene, named BASS2, for bile acid : sodium symporter family protein 2, the transcript of which is abundant in C4 plants that have the sodium-dependent pyruvate transporter. Recombinant BASS2 shows sodium-dependent pyruvate uptake activity.