Analysis of membrane fusion process and related proteins on cytokinesis of brown algae

在这项研究中，通过电子断层扫描进行了三维分析，以了解在细胞分裂过程中的过渡膜结构。分析表明，高尔基体衍生囊泡与扁平池（FCs）融合形成了复杂的带条纹结构（TA；管状附属物）。每个FC由TAs连接，形成膜性网络（MN）。细胞质分裂晚期，MN发育成宽膜囊。将三维图像转换为任意角度，表明从MN到MSc的膜融合过程分层。此外，利用二维凝胶电泳（2-DE）分离了经latrunculin B处理的受细胞质分裂和细胞质分裂阻断的合子中提取的蛋白质。在凝胶上可重复分离的约600个点中，来自细胞分裂阻断的受精卵的2- de与对照相比显示出31个低表达蛋白和2个高表达蛋白。

In this study, a three dimensional analysis was performed by electron tomography in zygotes of Silvetia babingtonii, for understanding the transitional membrane configuration during cytokinesis. The analysis revealed that a complicated architecture with fringes (TA; tubule-like appendage) were formed by fusion of Golgi derived vesicles to flat cisternae (FCs). Each FC was connected by TAs, thus forming the membranous network (MN). At advanced cytokinesis, the MN grew into broad membranous sac (MS). To turn the 3D image to arbitrary angle indicated a stratification of membrane fusion process from the MN to the MSc. Moreover, proteins extracted from zygotes of Fucus distichus on cytokinesis and cytokinesis blocked zygotes treated with latrunculin B were separated by two-dimensional gel electrophoresis (2-DE). From a total of about 600 spots reproducibly separated on gels, 2-DE from cytokinesis blocked zygotes showed 31 under-expressed and 2 over-expressed proteins in comparison with control.