Analysis of the infuluence of physiological and morphological variability to the synaptic integration of a nonspiking interneuron

本研究的目的是调查的影响，生理形态变异的突触整合（突触输出）的一个已确定的nonspiking interneuron（LDS神经元）使用神经生理学和计算方法。2009年，为了量化树突的膜电导和LDS细胞的三维结构的可变性，对它们的膜电导进行了神经生理学和神经生物学研究，并使用激光扫描显微镜测量了它们的三维结构。结果显示跨细胞的膜电导的大的可变性。然而，不能获得细胞的形态，同时使用细胞填充荧光染料荧光黄记录膜电导，使得不能从电生理记录的细胞定量细胞的三维结构。2010年，对LDS细胞中的三种类型的膜电导进行了定量估计。

The present study aimed to investigate the influence of physiological morphological variability to the synaptic integration (synaptic output) of an identified nonspiking interneuron (LDS neuron) using neurophysiological and computational methods. In 2009, in order to quantify the variability of the membrane conductance of dendrite and three-dimensional structure of the LDS cells, their membrane conductances were investigated neurophysiologically and neuropharmacologically and their three-dimensional structures were measured using a laser scanning microscope. The result shows large variability of the membrane conductance across the cells. However, the morphology of the cell could not be obtained while the membrane conductance was recorded at the same time using cell filling fluorescent dye, Lucifer Yellow so that three dimensional structures of the cells could not be quantified from the cell recorded electrophysiologically. In 2010, three types of membrane conductances in the LDS cell was estimated quantatively.