Investigation about the behavior of supramolecular complex in cell membrane : mechanism

构建了GFP-flin、Flim-GFP和GFP-FliG的表达系统，并证实了GFP-FliG的功能整合。在GFP荧光标记的马达中，观察了消逝光漂白后的荧光恢复情况。这是第一个证明在正常工作的细菌鞭毛马达中交换转子组件的证据。通过结合高速摄像机和微珠测定法来旋转单个大肠杆菌细胞的马达。这项实验表明，细胞上两个不同马达的开关是由细胞内信号蛋白--磷酸化的Chey协调控制的。在此基础上，提出了细胞内信号的波状传播模型。

Expression systems for GFP-FliN, FliM-GFP, and GFP-FliG were constructed, and the functional incorporation was confirmed. In a fluorescently labeled motor by GFP, the recovery of fluorescence after photobleaching by the evanescent light was observed. This is the first evidence for the exchange of rotor components in a functioning bacterial flagellar motor. The rotation of motors of individual E. coli cells by combining a high-speed camera and the bead assay method. From this experiment, it was showed that the switching of two different motors on a cell is controlled coordinatedly by an intracellular signal protein, phosphorylated CheY. From these results, wave-like propagation model of intracellular signal was proposed.