Analysis of the relationship between herbicide resistance mechanism and biophoton generation

分析了水稻培养细胞对除草剂敏感的光子发射的产生机理。50-100 ppm苯磺隆（BSM）处理诱导水稻细胞的光子发射增加。供试的18种除草剂的释放模式各不相同。从水稻细胞中分离到一个细胞色素P450基因CYP 81 A6，该基因被报道与水稻对BSM的耐受性有关。CYP 81 A6沉默细胞对BSM和支链氨基酸的反应表明，细胞色素P450对BSM的解毒反应参与了水稻细胞对BSM的响应性光子发射。

The generating mechanism of herbicide responsive photon emission from cultured rice cells was analyzed. 50-100 ppm bensulfronmethyl (BSM) treatment induced an increment of photon emission from rice cells. Emission patterns of 18 tested herbicides were different from each other. CYP81A6, a cytochrome P450 gene reported to be responsible for BSM tolerance in rice, was isolated from the rice cells, and was silenced by RNAi. Responses of CYP81A6-silenced cell line to BSM and branched chain amino acids revealed that the BSM detoxifying reaction by cytochrome P450 is involved in BSM responsive photon emission from rice cells.