The establishment of germ-free fish in guppy Poecilia reticulates

无菌（GF）动物是指没有微生物或寄生虫生活在其体内或体表的动物。本研究以卵生孔雀鱼（Poecilia retic网状孔雀鱼）为原料，建立GF鱼（GFF），并与常规组（CF）进行体生长和淋巴组织的比较。获得的GFF可以饲养100天以上，两组的体生长情况不同。与CF相比，GFF在肠道固有层和肾脏组织中分布的白细胞数量也有所减少。这些结果表明，CFF是分析含免疫调节的鱼类代谢与水生微生物关系的良好工具。

Germ-free(GF) animals are animals that have no microorganisms or parasites living in or on it. In the present study, we established the GF fish(GFF) from ovoviviparity fish guppy Poecilia reticulate, and compared the body growth and lymphoid tissues of GFF and conventional fish(CF) groups. Rearing of GFF obtained was possible for over 100 days and the body growth differed in the two groups. GFF also have a reduced number of leucocytes distributed in the lamina propria of intestine and kidney tissues compared with CF. These results indicate that the CFF is an excellent tool for analyzing of the relationship between fish metabolism containing immune modulation and aquatic microorganism.