Obstruction of HA antigen cleavage of influenza virus with recombinant H chain antibody and its antiviral effects

研制骆驼科动物的H链抗体是一项研究，据报道具有良好的抗原特异性和相容性，能有效地控制流感病毒HA蛋白，并为该病毒性疾病的治疗提供模型。用HA抗原免疫美洲驼后，在90℃下处理30分钟后，可分离出与HA抗原反应的H链抗体。将扩增的VHH抗体基因PCR产物导入噬菌粒载体，并从转化的DH5α中分离。有必要研究通过人工突变导入或DNaseI处理进行基因重组以分离具有更强亲和力的VHH克隆的效用。

It is a research that develops H chain antibody of the camel family animal, reported to have an excellent antigen specificity and compatibility, to control influenza virus HA protein effectively, and that provide the treatment model of the virus disease. The H chain antibodies were separated from the llama immunized with HA antigen, and were reactive to the HA antigen after the treat for 30 minutes at 90℃. The amplified VHH antibody gene PCR products were introduced into phagemid vectors and separated from transformed DH5α. It is necessary to examine the utility of the gene restructuring, by an artificial mutation introduction or the DNaseI processing, to isolate the VHH clone with a stronger affinity.