Development of oral inactivated recombinant Salmonella vaccine using E.coli cell surface display technique

为了研制口服肠炎沙门氏菌灭活疫苗，本研究构建了大肠杆菌表面展示质粒载体。MisL的n端结构域被归类为沙门氏菌V型分泌系统，作为锚定蛋白用于重组蛋白的表面展示。全功能霍乱毒素B亚基（CTB）与肠炎沙门氏菌疫苗候选蛋白的融合蛋白能够在大肠杆菌表面显示。用灭活的大肠杆菌表达的融合蛋白口服免疫鸡。肠道黏液中ctb特异性IgA滴度增强。本研究结果可用于研制简单、安全的口服SE疫苗。

In order to develop oral inactivated Salmonella Enteritidisvaccine, tne E. coli surface display plasmid vector was newly constructed in this study. The N-terminal domain of MisL, which is classified as Salmonella type V secretion system, was employed an anchor protein for surface display of recombinant protein. The fusion proteins of fully functional cholera toxin B subunit (CTB), which is one of the suitable candidates for mucosal adjuvant, and Salmonella Enteritidis vaccine candidate proteins were able to be displayed on the surface of E. coli. The chickens were immunized orally with the inactivated E. coli expressed fusion proteins. The CTB-specific IgA titers in intestinal mucus were enhanced. Our findings obtained in this study can be applied to development of simple and safety oral SE vaccine.