The functional analysis of autism related and BDNF secretion regulated protein CAPS2

脑源性神经营养因子（brain derived neurotrophic factor，BDNF）在神经发生、神经元存活和发育以及突触发育和可塑性中起着重要作用。然而，BDNF分泌的机制在很大程度上是未知的。最近，已发现Ca^2+依赖性分泌激活蛋白2（CAPS2）是调节BDNF分泌的蛋白质。在本研究中，我们证明CAPS2不仅加速BDNF分泌动力学，而且增加BDNF的量子释放，通过使用时间推移BDNF分泌成像技术。此外，CAPS2调节的BDNF分泌在GABA能中间神经元网络的发育中起关键作用。GABA能中间神经元损伤影响突触可塑性和焦虑样行为。

Brain derived neurotrophic factor (BDNF) plays critical role in neurogenesis, neuronal survival and development and in synaptic development and plasticity. However, the mechanism of the BDNF secretion is largely unknown. Recently, Ca^<2+> dependent activator protein for secretion 2 (CAPS2) has been discovered as a protein which regulates BDNF secretion. In the present study, we demonstrated that CAPS2 not only accelerates BDNF secretion kinetics but also increases BDNF quantal release by using time-lapse BDNF secretion imaging technique. In addition, CAPS2 regulated BDNF secretion plays a critical role in development of GABAergic interneuron network. The GABAergic interneuron impairments affect synaptic plasticity and anxiety-like behavior.