Isolation and characterization of a human monoclonal antibody against M2 of influenza A virus for reduction of avian influenza

在这项研究中，我们应用ISAAC方法检测了接种流感HA疫苗的健康志愿者外周血中流感病毒特异性b细胞，以产生人流感单克隆抗体。我们获得了许多与流感病毒反应的人单克隆抗体，并鉴定了它们的功能。其中一个（a1 -10）特异性结合基质蛋白2 （M2e）的外部结构域。研究表明，Ab1-10不仅对季节性甲型流感病毒有反应，而且对2009年H1N1大流行病毒（2009 H1N1）和高致病性甲型禽流感病毒也有反应。更重要的是，它在体外抑制了2009 H1N1病毒的复制，这表明它可能是一种强有力的医学应用候选者。

In this study, we applied ISAAC method to detect influenza virus-specific B-cells in peripheral blood from healthy volunteers who had been immunized with influenza HA vaccine to generate human influenza monoclonal antibodies. We obtained many human monoclonal antibodies reacting influenza viruses, and characterized their functions. One of them (Ab1-10) specifically bound external domain of the matrix protein 2 (M2e). We demonstrate that Ab1-10 reacted not only to seasonal influenza A viruses, but also to pandemic (H1N1) 2009 virus (2009 H1N1) and highly pathogenic avian influenza A virus. More importantly, it inhibited 2009 H1N1 viral replication in vitro, suggesting that it could be a potent candidate for medical application.