標準化半定量Real-time PCRを用いた呼吸器感染症の包括的迅速診断法

共生微生物是肺炎的常见原因。然而，检测到呼吸道中的硒微生物并不意味着它们是致病原。Hira-Tan是一种基于实时聚合酶链式反应的系统，它能够在痰中检测到的共生微生物中识别应该作为治疗靶标的致病菌。我们成功地提出了临界值，该临界值可以区分淋巴靶点和非治疗靶点，即使在PCR检测时也是如此。HI RA-TAN还针对致病的非共生微生物，使其本身成为鉴定肺炎病原体的综合试验。我们采用了一项前瞻性的研究来调查Hira-Tan的性能。结论：Hira-Tan是一种敏感的检测肺炎病原菌的方法，可为肺炎患者选择合适的治疗方案提供有价值的信息。

Commensal organisms are frequent causes of pneumonia. However, the detection of the se organisms in the airway does not mean that they are the causative pathogens. The HIRA-TAN is a real-time PCR-based system which has the ability to identify the path ogens that should be therapeutically targeted among the commensal organisms detected in the sputum. We successfully developed the cutoff value, which differentiate The rapeutic targets and Non-therapeutic target even when it is detected by PCR. The HI RA-TAN also targets the pathogenic non-commensal organisms, making itself a comprehe nsive test for the identification of the pathogens of pneumonia. We employed a pros pective study to investigate the performance of the HIRA-TAN. We concluded that the HIRA-TAN is a sensitive method to identify the causative organism in pneumonia and provide valuable information to choose an appropriate treat the pneumonic patient.